RBRC05765 true 理化学研究所免疫 アレルギーセンター 古関明彦先生。M1 ES細胞を用いて作出。Neoは除去済み。 FLP/frt system B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko> B6.Cg-H2az1<tm1.1Hko> H2az2<tm1.1Hko> The RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Necessary documents for ordering:<ol><li>Order form (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx">English</A>)</li><li>Category I MTA: MTA for distribution with RIKEN BRC (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx">English</A>)</li><li>Acceptance of responsibility for living modified organism (<A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx">Japanese</A> / <A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx">English</A>)</li></ol> Cre/loxP system H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl] H2afz : Homozygote x Homozygote ; H2afv : Homozygote x Homozygote [or Crossing to C57BL/6JJcl] <a href='https://brc.riken.jp/mus/pcr05765'>Genotyping protocol -PCR-</a> P1 Phage loxP sites, yeast FRT (flipase recombination target) site, mouse H2az1 genomic DNA, mouse H2az2 genomic DNA M1 [(C57BL/6 x 129)F1] C (3-6 months) C（3〜6か月） Haruhiko KOSEKI 条件を付加する。<br>利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。 H2A.Zのfloxedマウス。H2A.Z1/2は、哺乳類細胞のクロマチンに恒常的に存在するヒストン変異体。この遺伝子を欠損したマウスは胎生致死 (E6.5) となることから、哺乳類の発生、生存に必須なヒストンであると考えられる。クロマチン内でのエピジェネチックな遺伝子制御機構の解明に有用。 古関　明彦 H2AZ1/2 floxed, B6-H2AZ1/2 floxed H2AZ1/2 floxed, B6-H2AZ1/2 floxed Developed by Dr. Haruhiko Koseki, RIKEN Center for Allergy and Immunology in 2009. M1 ES cells derived from (C57BL/6 x 129) F1 were used. The mutant mice were crossed to C57BL/6J. Neomycin resistant genes were removed. H2A.Z1 and Z2 floxed mice. Histone variant H2A.Z (H2afz) gene is required for mammalian development.